These two methods are all based on phage display of humanized scFv variants and selection of high-affinity humanized binders through bio-panning. The first approach is CDR grafting and the second is chain shuffling. As a means of circumventing these problems, we have developed three antibody humanization strategies that can preserve the specificity and affinity of the antibody toward the antigen whereas significantly or completely eliminate the immunogenicity of the antibody in humans. Importantly, they are rapidly removed from circulation and can cause systemic inflammatory effects as well. Rodent antibodies are highly immunogenic in humans, which limits their clinical applications, especially when repeated administration is required. Since the development of the hybridoma technology, a large number of rodent monoclonal antibodies with specificity for antigens of therapeutic interest have been generated and characterized. Humanization is important for reducing the immunogenicity of monoclonal antibodies derived from xenogeneic sources (commonly rodent) and for improving their activation of the human immune system. We also provide humanization service to antibodies derived from other species, such as non-human primate (NHP), rabbit, dog, chicken, llama and etc. We have successfully performed 15 mouse/rat humanization projects during the past decade with at least one humanized antibody entered the clinical trials. HighlightsĬreative Biolabs has extensive experience to offer the antibody humanization service for therapeutic and diagnostic development. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects. Cell-based Membrane Protein Expression and PurificationĬreative Biolabs is offering the most comprehensive services for antibody development projects.Cell-free Membrane Protein Expression and Purification.Membrane Protein Solubilization and Stabilization.Manufacturability Assessment and Optimization.SIAT ® Immunogenicity Assessment Services.CreMap™ Epitope Mapping & Discovery Services.Dual-Polarization Interferometry (DPI) Services.Differential Scanning Calorimetry (DSC) Services.Isothermal Titration Calorimetry (ITC) Services.De Novo Human/Humanized Antibody Sequencing Service.Magic™ Human Antibody Production Using Transgenic Mice.Bovine Ultralong CDR3 Antibody Humanization.Antibody Off-target Identification and Specificity Profiling Service.Biomolecular Interaction Analysis Services.Epitope Prediction for the Identification of Antibody Epitopes.Epitope Mapping by X-ray Crystallography.Phage Display & Antibody Library Services.
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